Limit of detection for a bead-based diffraction biosensor

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Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications Vii 7574,(2010)


Diffraction-based biosensors that rely on optical scattering are a sensitive approach for biomolecular detection. We present a Mie surface double-interaction computer study of the in situ assembly of bead-based diffraction gratings on a gold substrate. The limit of detection for a system having 26 stripes is calculated to be approximately 25 beads in total, or approximately one bead per stripe in an immunoassay experiment. This sensitivity limit is orders of magnitude better than label-free molecular sensors, and is consistent with high-speed scanning for high-throughput assays.

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