Date of Award
Spring 2015
Degree Type
Thesis
Degree Name
Master of Science in Biomedical Engineering
Department
Biomedical Engineering
First Advisor
Sherry Voytik-Harbin
Committee Chair
Sherry Voytik-Harbin
Committee Member 1
Corey Neu
Committee Member 2
Stacey Halum
Abstract
Collagen has long been used as a material for tissue engineering due to its prevalence in the extracellular matrix of connective tissues. However, traditional collagen materials utilizing atelocollagen and acid solubilized telocollagen have lacked the mechanical integrity and collagen fibril density found in the in vivo state. Here, we utilize collagen oligomers and confined compression to forcibly remove a portion of the fluid phase component. Materials were created with controlled, substantially increased material properties, including order of magnitude increases in collagen fibril density, elastic modulus, compressive modulus,and resistance to proteolytic degradation. The technique was found to be amenable to cell encapslation, allowing the creation of viable cellularized high density constructs. The translational aspects of these high density collagen materials was examined through the ultrastructure and bulk property changes that may be present through lyophilization and rehydration, as well as comparison against a gold standard market crosslinked microfibrillar collagen sponge.
Recommended Citation
Blum, Kevin, "High density collagen fibril constructs with tunable mechano-biology in acellular and cellular configurations" (2015). Open Access Theses. 553.
https://docs.lib.purdue.edu/open_access_theses/553