Title
1,25-Dihydroxyvitamin D Regulation of Triacylglycerol Accumulation in Differentiated Adipocytes
Date of Award
January 2016
Degree Type
Dissertation
Degree Name
Doctor of Philosophy (PhD)
Department
Nutrition Science
First Advisor
Dorothy Teegarden
Committee Member 1
Kimberly K Buhman
Committee Member 2
Shawn S Donkin
Committee Member 3
Kee-Hong Kim
Committee Member 4
Shihuan Kuang
Abstract
Obesity is a major public health concern, both in the United States and worldwide. Therefore, identification of measures by which obesity may be prevented and reversed is of high priority. Epidemiological studies consistently demonstrate an inverse relationship between serum 25(OH)D levels, an indicator of vitamin D status, and measures of adiposity. These data suggest that vitamin D may play a role in the prevention of excessive adiposity. However, whether vitamin D impacts lipid storage and metabolism in terminally differentiated adipocytes is not yet known. The purpose of this work was to determine the impact of 1,25-dihydroxyvitamin D (1,25(OH)2D), the bioactive vitamin D metabolite, on triacylglycerol accumulation and lipid and glucose metabolism in differentiated adipocytes. To study this, 3T3-L1 adipocytes were differentiated for 9 days, followed by stimulation with 1,25(OH)2D (10 nM) or vehicle for 1-7 days. Results indicate that 1,25(OH)2D stimulates a 21% reduction in TAG accumulation in differentiated 3T3-L1 adipocytes after 4 days (P=0.01). This occurs despite a significant increase in fatty acid uptake (P<0.01), assessed using BODIPY FL C16, and with concomitant stimulation of PKA-dependent
Recommended Citation
Larrick, Brienna, "1,25-Dihydroxyvitamin D Regulation of Triacylglycerol Accumulation in Differentiated Adipocytes" (2016). Open Access Dissertations. 1279.
https://docs.lib.purdue.edu/open_access_dissertations/1279