The authors report a technique which produces cell-to-cell electrofusion using a common direct current power supply on a microfluidic platform. In the authors' method, the cells were first conjugated based on biotin-streptavidin interaction. The electrofusion was then conducted by flowing the linked cells through a simple microfluidic channel with geometric variation under continuous direct current voltage. This microfluidics-based technique offers processing at the level of single cell pairs with efficiency comparable to that of conventional electrofusion technique based on electrical pulses. (c) 2006 American Institute of Physics.
ELECTROFUSION; ELECTROPHORESIS; HYBRIDIZATION; DEVICE
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