Surface topography and chemistry shape cellular behavior on wide band-gap semiconductors

Lauren E. Bain, North Carolina State University, University of North Carolina
Ramon Collazo, North Carolina State University at Raleigh
Shu-han Hsu, Purdue University, Birck Nanotechnology Center
Nicole Pfiester Latham, Purdue University
Michael J. Manfra, Purdue University, Birck Nanotechnology Center
Albena Ivanisevic, North Carolina State University at Raleigh

Date of this Version



The chemical stability and electrical properties of gallium nitride make it a promising material for the development of biocompatible electronics, a range of devices including biosensors as well as interfaces for probing and controlling cellular growth and signaling. To improve the interface formed between the probe material and the cell or biosystem, surface topography and chemistry can be applied to modify the ways in which the device interacts with its environment. PC12 cells are cultured on as-grown planar, unidirectionally polished, etched nanoporous and nanowire GaN surfaces with and without a physi-sorbed peptide sequence that promotes cell adhesion. While cells demonstrate preferential adhesion to roughened surfaces over as-grown flat surfaces, the topography of that roughness also influences the morphology of cellular adhesion and differentiation in neurotypic cells. Addition of the peptide sequence generally contributes further to cellular adhesion and promotes development of stereotypic long, thin neurite outgrowths over alternate morphologies. The dependence of cell behavior on both the topographic morphology and surface chemistry is thus demonstrated, providing further evidence for the importance of surface modification for modulating bio-inorganic interfaces. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.


Nanoscience and Nanotechnology