A humidity-sensitive hydrogel-Bacillus spore composite for micropatterning of biomolecular gradients

Richard L. Gieseck, Birck Nanotechnology Center, Purdue University
Bin-Da Chan, Birck Nanotechnology Center, Purdue University
Cagri A. Savran, Birck Nanotechnology Center, Purdue University

Date of this Version



Copyright 2013 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Rev. Sci. Instrum. 84, 085003 (2013); and may be found at http://dx.doi.org/10.1063/1.4817971. The following article has been submitted to/accepted by Review of Scientific Instruments. Copyright 2013 Richard L. Gieseck, Bin-Da Chan and Cagri A. Savran. This article is distributed under a Creative Commons Attribution 3.0 Unported License.


A composite material consisting of Bacillus subtilis spores suspended in a humidity sensitive hydrogel can be used to pattern biomolecules in different concentrations directly onto glass surfaces using a mechanical micromanipulator. By altering the relative humidity surrounding the composite gel during deposition, surface concentration of patterned biomolecules can be controlled and varied to create user-defined, biomolecular surface concentrations. (C) 2013 AIP Publishing LLC.


Nanoscience and Nanotechnology