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Proposal

Manganese, an essential trace mineral, plays crucial roles in various physiological processes within living organisms. However, excessive exposure to manganese can lead to toxicity, particularly impacting the central nervous system. This study aimed to assess the impact of manganese exposure on locomotor behavior during early development using zebrafish (Danio rerio) as a model organism. Zebrafish embryos at 1–2 hours post fertilization (hpf) were exposed to five different concentrations of manganese chloride (0, 0.01, 0.05, 0.1, or 0.5 mM) until 120 hpf. Locomotor behavior was assessed using the visual motor response assay, measuring parameters such as total distance moved, velocity, and time spent moving. Additional assessments included spontaneous movement at 24 hpf, heart rate at 48 hpf, and survival and hatching rates from 24 to 120 hpf. Results indicated significant alterations in locomotor behavior, with decreased total distance moved and velocity observed in higher concentration groups during dark phases. Interestingly, the lowest concentration group exhibited hyperactivity during light phases, suggesting a dose-dependent effect on behavior. Spontaneous movement significantly increased at 0.05 mM concentration, while heart rate decreased at the highest concentration. Hatching rates were elevated in the two highest concentration groups at 24 hpf. These findings underscore the association between manganese exposure and locomotor behavioral changes during early development. Moreover, the differential effects observed across concentration groups suggest varying mechanisms underlying manganese toxicity. Zebrafish serve as a valuable model for investigating developmental neurotoxicity, providing insights relevant to human health outcomes.

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