Recommended Citation
Patel, Shivan H.; Yue, Feng; Saw, Shannon K.; Foguth, Rachel; Cannon, Jason R.; Shannahan, Jonathan H.; Kuang, Shihuan; Sabbaghi, Arman; and Carroll, Chad C., "Advanced Glycation End-Products Suppress Mitochondrial Function and Proliferative Capacity of Achilles Tendon-Derived Fibroblasts" (2019). Purdue University Libraries Open Access Publishing Fund. Paper 125.
http://dx.doi.org/10.1038/s41598-019-49062-8
DOI
10.1038/s41598-019-49062-8
Date of this Version
8-30-2019
Abstract
Debilitating cases of tendon pain and degeneration affect the majority of diabetic individuals. The high rate of tendon degeneration persists even when glucose levels are well controlled, suggesting that other mechanisms may drive tendon degeneration in diabetic patients. The purpose of this study was to investigate the impact of advanced glycation end-products on tendon fibroblasts to further our mechanistic understanding of the development and progression of diabetic tendinopathy. We proposed that advanced glycation end-products would induce limitations to mitochondrial function and proliferative capacity in tendon-derived fibroblasts, restricting their ability to maintain biosynthesis of tendon extracellular matrix. Using an in-vitro cell culture system, rat Achilles tendon fibroblasts were treated with glycolaldehyde-derived advanced glycation end-products (0, 50, 100, and 200 μg/ml) for 48 hours in normal glucose (5.5 mM) and high glucose (25 mM) conditions. We demonstrate that tendon fibroblasts treated with advanced glycation end-products display reduced ATP production, electron transport efficiency, and proliferative capacity. These impairments were coupled with alterations in mitochondrial DNA content and expression of genes associated with extracellular matrix remodeling, mitochondrial energy metabolism, and apoptosis. Our findings suggest that advanced glycation end-products disrupt tendon fibroblast homeostasis and may be involved in the development and progression of diabetic tendinopathy.
Comments
This is the publisher PDF of Patel, S.H., Yue, F., Saw, S.K. et al. Advanced Glycation End-Products Suppress Mitochondrial Function and Proliferative Capacity of Achilles Tendon-Derived Fibroblasts. Scientific Reports 9, 12614 (2019). This article is distributed under a CC-BY license, and is available at DOI: 10.1038/s41598-019-49062-8.