The aim of this study was, firstly, to evaluate the phenol profile of thistle (Cirsium japonicum, CJ) by High performance liquid chromatography-electrospray ionization–mass spectrometry (HPLC–ESI–MS), dried by different methods (90 ◦C hot-air, 70 ◦C hot-air, shade-, and freeze-drying). Secondly, we aimed to evaluate the relationship between phenolic compounds content and antioxidant properties. CJ contained chlorogenic acid, linarin, and pectolinarin. Total phenolic contents of CJ significantly decreased under hot-air-drying condition, especially chlorogenic acid contents in CJ have been reduced by 85% and 60% for 90 ◦C and 70 ◦C hot-air-drying, respectively. We evaluated the protective effect on adrenal pheochromocytoma (PC12) cells and Caenorhabditis elegans using shade-dried CJ, which has the largest phenolic contents and the strongest antioxidant property. CJ-treated PC 12 cells dose-dependently exhibited the protective effects against reactive oxygen species (ROS), while cell viability increases, lactate dehydrogenase release decreases, and ROS formation decreases. Furthermore, CJ has also shown protection against ROS in C. elegans. Consequently, CJ contributed to lifespan extension under ROS stress without influencing the physiological growth.


This is the publisher's version of Jang Miran, Zhang Yuan, Bai Juan, Jun-Bae An, Park Yeonhwa, Kim Gun-Hee, Kee-Hong Kim, Piceatannol, a Natural Stilbene, Extends the Lifespan of Caenorhabditis elegans Under Fasting Through Inhibition of Lipolysis, Current Developments in Nutrition, Volume 4, Issue Supplement_2, June 2020, Page 38, https://doi.org/10.1093/cdn/nzaa040_038


thistle (Cirsium japonicum); phenolic compounds; antioxidant; reactive oxygen species (ROS); PC 12 cells; Caenorhabditis elegans

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