A set of eight microsatellite markers was used to genotype 25 black walnut (Juglans nigra L.) clones within the Purdue University germplasm repository. The identities of 212 ramets were verified using the same eight microsatellite markers. Some trees were mislabeled and corrected as to clone using analysis of microsatellite markers. A genetic dendrogram was constructed to show the degree of genetic relatedness between clones. Two additional dendrograms, one based on crown architecture traits and the other on tree size and form traits, were also built and compared with the genetic dendrogram. The genetic dendrogram showed that these eight molecular markers had the ability to distinguish genetically related clones from less related ones. Crown architecture traits and tree size and form traits were able to group genetically related clones together, but less accurately than the genetic matrix.


Pang, K., Woeste, K., Michler, C. 2017. Cultivar Identification and Genetic Relatedness among 25 Black Walnut (Juglans nigra L.) Clones Based on Microsatellite Markers. IN: In: Kabrick, John M.; Dey, Daniel C.; Knapp, Benjamin O.; Larsen, David R.; Shifley, Stephen R.; Stelzer, Henry E., eds. Proceedings of the 20th Central Hardwood Forest Conference; 2016 March 28-April 1; Columbia, MO. General Technical Report NRS-P-167. Newtown Square, PA: U.S. Department of Agriculture, Forest Service, Northern Research Station: 167- 182

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