Effect of acute aerobic and resistance exercise on skeletal muscle metabolic enzyme activity

Jessica Susanne Solfest, Purdue University


The spreading obesity epidemic and associated risk of diabetes necessitates exploration for avenues of treatment. A known method to prevent and offset effects of type II diabetes is exercise. Both aerobic and resistance training increase metabolism and concurrent exercise training improves glycemic control further than either exercise modality alone, but little is known about metabolism following acute exercise. Key enzymes regulating cellular metabolic pathways include phosphofructokinase (PFK), 3-L-hydroxyacyl CoA dehydrogenase (β-HAD), citrate synthase (CS), and succinate dehydrogenase (SDH). Observing the responses of these enzymes to acute exercise may indicate the rate of metabolism and preferential fuel sources. In this study, twelve healthy, non-obese, sedentary males performed acute aerobic exercise on a cycle ergometer followed by acute single leg resistance exercise on a knee extensor machine. Skeletal muscle enzyme activities were measured before and 1 hour after acute exercise to analyze the effects of each modality. There were no changes in PFK, β-HAD, CS, or SDH activities in response to acute aerobic or aerobic + resistance exercise. Further research is necessary to develop a greater understanding of metabolic activity associated with acute exercise and to potentially enhance treatment methods for metabolic diseases.




Gavin, Purdue University.

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