Impact of inflammation on gene expression in a prostate cancer model
Prostate cancer is one of the most common malignant cancers in men in Western countries. More and more recent studies have demonstrated a link between chronic inflammation and progression of cancers. To study the impact of prostate inflammation on the development of prostate cancer, we developed a POET3-PTEN mouse model. This mouse model expresses membrane bound ovalbumin (mOVA) on prostate epithelial cells and either PTEN null, PTEN heterozygoutes or PTEN wild type. After inflammation was induced by injection of mOVA specific CD8 T cells (OT-I) in POET3-PTEN mice, immunohistochemical analysis was performed to evaluate molecular expression in prostate tissues. This study demonstrated that expression of NKX3.1 and p63 was influenced by induced and spontaneous inflammation. The expression pattern of E-cadherin was not changed in POET3-PTEN +/+, POET3-PTEN+/- and POET3-PTEN-/- mice. N-cadherin expression was only detected in POET3-PTEN-/- mice. Ki-67 staining showed increased proliferative index after inflammation was induced in prostate. This study provided a molecular link underlying the prostate inflammation and prostate cancer development.
Ratliff, Purdue University.
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