SWI/SNF Chromatin Remodeling Complexes in Porcine Embryos
Gene expression is under precise regulation during early mammalian embryonic development. Epigenetic modifications are a major aspect of studying gene expression. SWI/SNF chromatin remodeling complexes are large multiprotein complexes, which can reposition nucleosomes and alter transcription factor accessibility to DNA. Subunits of SWI/SNF chromatin remodeling complexes play essential roles in development by guiding the complexes to specific loci. The aims of this study were to 1) determine the function of ARID1A, a SWI/SNF chromatin remodeling complex subunit during cleavage development in the porcine embryo and 2) determine the intracellular localization of four SWI/SNF subunits, ARID1A, ARID2, BRD7, and SNF5, in porcine embryos using an immunocytochemical approach. Our data show that ARID1A is essential for early embryo development. Our results indicate that individual SWI/SNF subunits adopt unique intracellular localization patterns at discrete developmental time points. Together, our data suggest that the collection of SWI/SNF complexes within blastomeres of cleavage stage embryos may differ at discrete developmental stages.
Cabot, Purdue University.
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