Epigenetic Regulation of Gene Expression through DNA Hydroxymethylation
The epigenetic regulation of gene expression was explored by probing the effect of methylation and hydroxymethylation on granulopoiesis. In order to pursue this end, hMeDIP-seq, MeDIP-seq, and RNA-seq were performed on undifferentiated and DMSO-differentiated HL-60 cells. This allowed us to compare locations of genomic methylation and hydroxymethylation to gene expression. HL-60 cells are a form of acute myeloid leukemia, and a better understanding of its mechanisms could lead to therapeutic targets. This study identified key changes in methylation and hydroxymethlation in pathways critical to granulpoiesis, and observed changes in transcript level in genes associated with these changes in methyl state.
Kirchmaier, Purdue University.
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