Abstract
Eighty-eight rice (Oryza sativa) cDNAs encoding rice leaf expressed protein kinases (PKs) were fused to a Tandem Affinity Purification tag (TAP-tag) and expressed in transgenic rice plants. The TAP-tagged PKs and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by tandem mass spectrometry. Forty-five TAP-tagged PKs were recovered in this study and thirteen of these were found to interact with other rice proteins with a high probability score. In vivo phosphorylated sites were found for three of the PKs. A comparison of the TAP-tagged data from a combined analysis of 129 TAP-tagged rice protein kinases with a concurrent screen using yeast two hybrid methods identified an evolutionarily new rice protein that interacts with the well conserved cell division cycle 2 (CDC2) protein complex.
Date of this Version
8-19-2009
DOI
10.1371/journal.pone.0006685
Recommended Citation
Rohila, Jai S.; Chen, Mei; Chen, Shuo; Chen, Johann; Cerney, Ronald L.; Dardick, Christopher; Canlas, Patrick; Fujii, Hiroaki; Gribskov, Michael; Kanrar, Siddhartha; Knoflicek, Lucas; Stevenson, Becky; Xie, Mingtang; Xu, Xia; Zheng, Xianwu; Zhu, Jian-Kang; Ronald, Pamela; and Fromm, Michael E., "Protein-Protein Interactions of Tandem Affinity Purified Protein Kinases from Rice." (2009). Department of Biological Sciences Faculty Publications. Paper 63.
http://dx.doi.org/10.1371/journal.pone.0006685
Comments
This is the publisher pdf of Rohila JS, Chen M, Chen S, Chen J, Cerny RL, et al. (2009) Protein-Protein Interactions of Tandem Affinity Purified Protein Kinases from Rice. PLoS ONE 4(8): e6685 and is available at: 10.1371/journal.pone.0006685.