Abstract
We describe a genetic system for producing specific gene knockouts inCyanothece sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a ΔnifK mutant, and it was unable to grow on medium lacking combined nitrogen and produced virtually no hydrogen.
Date of this Version
9-17-2010
DOI
10.1128/AEM.01456-10
Recommended Citation
Min, Hongtao and Sherman, Louis A., "Genetic Transformation and Mutagenesis Via ssDNA in the Unicellular, Diazotrophic Cyanobacteria of the Genus Cyanothece." (2010). Department of Biological Sciences Faculty Publications. Paper 32.
http://dx.doi.org/10.1128/AEM.01456-10
Comments
Copyright © American Society for Microbiology, [Applied and Environmental Microbiology, 76, 2010, 7641-7645 and 10.1128/AEM.01456-10