We describe a genetic system for producing specific gene knockouts inCyanothece sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a ΔnifK mutant, and it was unable to grow on medium lacking combined nitrogen and produced virtually no hydrogen.
Date of this Version
Min, Hongtao and Sherman, Louis A., "Genetic Transformation and Mutagenesis Via ssDNA in the Unicellular, Diazotrophic Cyanobacteria of the Genus Cyanothece." (2010). Department of Biological Sciences Faculty Publications. Paper 32.