Abstract
Transcriptome analysis of bovine mammary development has provided insight into regulation of mammogenesis. However, previous studies primarily examined expression of epithelial and stromal tissues combined, and consequently did not account for tissue specific contribution to mammary development. Our objective was to identify differences in gene expression in epithelial and intralobular stromal compartments. Tissue was biopsied from non-lactating dairy cows 3 weeks prepartum, cut into explants and incubated for 2 hr with insulin and hydrocortisone. Epithelial and intralobular stromal tissues were isolated with laser capture microdissection. Global gene expression was measured with Bovine Affymetrix GeneChips, and data were preprocessed using RMA method. Moderated t-tests from gene-specific linear model analysis with cell type as a fixed effect showed more than 3,000 genes were differentially expressed between tissues (P
Date of this Version
7-29-2011
DOI
10.1371/journal.pone.0022541
Recommended Citation
Casey, Theresa; Dover, Heather; Liesman, James; DeVries, Lindsey; Kiupel, Matti; VandeHaar, Michael; and Plaut, Karen, "Transcriptome Analysis of Epithelial and Stromal Contributions to Mammogenesis in Three Week Prepartum Cows." (2011). Department of Animal Sciences Faculty Publications. Paper 2.
http://dx.doi.org/10.1371/journal.pone.0022541
Comments
This is the publisher pdf of Casey T, Dover H, Liesman J, DeVries L, Kiupel M, et al. (2011) Transcriptome Analysis of Epithelial and Stromal Contributions to Mammogenesis in Three Week Prepartum Cows. PLoS ONE 6(7): e22541 and is available at: 10.1371/journal.pone.0022541.