Abstract
For starch digestion to glucose, two luminal α-amylases and four gut mucosal α-glucosidase subunits are employed. The aim of this research was to investigate, for the first time, direct digestion capability of individual mucosal α-glucosidases on cooked (gelatinized) starch. Gelatinized normal maize starch was digested with N- and C-terminal subunits of recombinant mammalian maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI) of varying amounts and digestion periods. Without the aid of α-amylase, Ct-MGAM demonstrated an unexpected rapid and high digestion degree near 80%, while other subunits showed 20 to 30% digestion. These findings suggest that Ct-MGAM assists α-amylase in digesting starch molecules and potentially may compensate for developmental or pathological amylase deficiencies.
Date of this Version
5-1-2012
DOI
10.1371/journal.pone.0035473
Recommended Citation
Lin, Amy Hui-Mei; Nichols, Buford L.; Quezada-Calvillo, Roberto; Avery, Stephen E.; Sim, Lyann; Rose, David R.; Naim, Hassan Y.; and Hamaker, Bruce R., "Unexpected High Digestion Rate of Cooked Starch by the Ct-Maltase-Glucoamylase Small Intestine Mucosal α-Glucosidase Subunit." (2012). Department of Speech, Language, and Hearing Sciences Faculty Publications. Paper 2.
http://dx.doi.org/10.1371/journal.pone.0035473
Comments
This is the publisher pdf of Lin AH-M, Nichols BL, Quezada-Calvillo R, Avery SE, Sim L, et al. (2012) Unexpected High Digestion Rate of Cooked Starch by the Ct-Maltase-Glucoamylase Small Intestine Mucosal α-Glucosidase Subunit. PLoS ONE 7(5): e35473 and is available at: 10.1371/journal.pone.0035473.