Patterned protein microarrays for bacterial detection
Langmuir 19,2 (2003) 436-439;
Patterned microarrays of antibodies were fabricated and tested for their ability to bind targeted bacteria. These arrays were used in a series of bacterial immunoassays to detect E. coli O157:147 and Renibacterium salmoninarum (RS). Microarrays were fabricated using microcontact printing (muCP) and characterized using scanning probe microscopy (SPM). The high-resolution SPM imaging showed that targeted bacteria had a higher binding selectivity to complementary antibody patterns than to unfunctionalized regions of the substrate. Additional studies indicated a significant reduction in binding of bacteria when the microarrays were exposed to nonspecific bacteria. These studies demonstrate how protein microarrays could be developed into useful platforms for sensing microorganisms.
self-assembled monolayers;; quartz-crystal microbalance;; cell detection;; gold;; fabrication;; diffraction;; microscopy;; salmonella;; features;; layers
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