Electrochemical glutamate biosensing with nanocube and nanosphere augmented single-walled carbon nanotube networks: a comparative study

Jonathan C. Claussen, Purdue University - Main Campus
Mayra S. Artiles, Purdue University
Eric S. McLamore, Purdue University
Subhashree Mohanty, Purdue University
Jin Shi, Purdue University
Jenna Rickus, Purdue University - Main Campus
Timothy S. Fisher, Purdue University
D. Marshall Porterfield, Purdue University - Main Campus

Abstract

We describe two hybrid nanomaterial biosensor platforms, based on networks of single-walled carbon nanotubes (SWCNTs) enhanced with Pd nanocubes and Pt nanospheres and grown in situ from a porous anodic alumina (PAA) template. These nanocube and nanosphere SWCNT networks are converted into glutamate biosensors by immobilizing the enzyme glutamate oxidase (cross-linked with gluteraldehyde) onto the electrode surface. The Pt nanosphere/SWCNT biosensor outperformed the Pd nanocube/SWCNT biosensor and previously reported similar nanomaterial-based biosensors by amperometrically monitoring glutamate concentrations with a wide linear sensing range (50 nM to 1.6 mM) and a small detection limit (4.6 nM, 3 sigma). These results combined with the biosensor fabrication scheme (in situ growth of SWCNTs, electrodeposition of metal nanoparticles, and facile enzyme immobilization protocol) create a biosensor that can potentially be scaled for integration into a wide range of applications including the treatment of neurological disorders.

Discipline(s)

Nanoscience and Nanotechnology