Biomagnetic Glasses: Preparation, Characterization, and Biosensor Applications

Yu-Ho Won, Purdue University - Main Campus
Ho Seong Jang, Purdue University - Main Campus
Seung Min Kim, Purdue University - Main Campus
E A. Stach, Birck Nanotechnology Center and School of Materials Engineering, Purdue University
Mallikarjunarao Ganesana, Clarkson University
Silvana Andreescu, Clarkson University
L Stanciu, Birck Nanotechnology Center and School of Materials Engineering, Purdue University

Abstract

In this work, a novel avenue to create a generic approach for the fabrication of biofunctional materials with magnetic capabilities to be used in the design of highly stable, magnetically separable enzyme-based systems was explored. As a model system, immobilization of acetylcholinesterase (AChE) was investigated using biomagnetic glasses composed of a magnetic core with a size tunable porous silica shell. The efficiency of the immobilization was determined by analyzing the biosensing capability of these biomagnetic glasses for the detection of the organophosphorous pesticide paraoxon. Screen printed electrodes with the AChE-biomagnetic glasses showed higher current response and stability than for the free enzyme. The detection limit of the paraoxon biosensor was in the nanomolar range.

Discipline(s)

Engineering | Nanoscience and Nanotechnology