Genetics of resistance to barley yellow dwarf virus in oat
Abstract
Barley yellow dwarf virus (BYDV) causes disease in many of the Poaceae and there are significant economic losses annually in oat (Avena sativa L.). The symptoms of virus infection are reddened leaves, Minted growth and blasted florets. A single gene resistance has not been characterised in oat. The objective was to determine the number of genes conditioning resistance in the oat line P88122-1, and to identify associated DNA markers. A recombinant inbred population was developed by single seed descent from two near-isogenic lines of P88122. The phenotypic evaluation of the recombinant inbred lines and (enzyme linked immunosorbent assay) ELISAs indicated that there was a single tolerance gene and at least one resistance gene. To determine the number of resistance genes in the fine P88122 F2 populations and F 3 families were developed from crosses between a resistant line and the susceptible cultivar Clintland 64. These populations were inoculated with P-PAV strain of BYDV in the field and greenhouse and the plants phenotyped at heading. It was determined that there were two resistance genes that were co-dominant and additive. Two F2 populations were developed that were segregating for unique single resistance genes. The amplified fragment length polymorphism. (AFLP) technique and bulked segregant analysis were used to identify a marker that is located 16.7cM from the BYDV resistance gene of P95145A1. This polymorphic band is also present in INO9201 that has a moderate level of resistance to BYDV.
Degree
Ph.D.
Advisors
Ohm, Purdue University.
Subject Area
Plant pathology|Genetics|Agronomy
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