A STUDY OF THE MORPHOLOGIC CHARACTER AND IN VIVO RADIOSENSITIZING EFFECT OF 5-THIO-ALPHA-D-GLUCOSE TOWARD A MALIGNANT MELANOMA OF GOLDEN SYRIAN HAMSTERS (RADIOLOGY, ONCOLOGY, FERTILITY)

BARRY MICHAEL BERNER, Purdue University

Abstract

Low LET radiation was evaluated alone and in combination with 5-thio-(alpha)-D-glucopyranose (5TDG) for reducing the in vivo growth of amelanotic malignant melanoma. Morphologic features of the tumor were determined at the light microscopic level.^ Percent tumor necrosis increased significantly when tumor volume exceeded the range 0.15-0.45 cm('3), but not for the ranges of 0.45-0.75 and 0.75-1.05 cm('3). The vascular density was constant within viable regions, but was quite variable in the necrotic areas.^ Pure 5TDG and the mother liquor were compared in regard to cytotoxic properties following oral administration for 22 days. Spermatogenesis was assayed qualitatively, while testis mass was used as a quantitative variable. Equal effects for identical dose levels were found for both variables.^ Nine groups of tumor-bearing animals received radiation and/or 5TDG treatments and a tenth group served as non-treatment controls. The radiation protocols, equivalent in terms of "Time-Dose-Fractionation" value, were 2.25, 5.20, or 8.00 gray per treatment. The 5TDG as mother liquor was administered orally for 22 days at 0.75 or 1.5 g/kg-day. The 1.5 g/kg-day dose level was omitted from a second study.^ In the first radiosensitization study, animals treated with radiation or radiation and 5TDG, exhibited a reduction in tumor growth compared to the control group. The group receiving 5.20 gray and 0.75 g/kg-day displayed a significant reduction in tumor growth for Days 8-22 of the study compared to the control group. Treatment with 5TDG alone did not reduce tumor growth.^ In the second study, treatment with radiation alone reduced tumor growth. Radiation and drug treatment protocols did not reproduce the findings from the first radiosensitization study.^ When the data from both experiments were examined utilizing analysis of variance (ANOVA) procedures, radiation, time, and blocks with 5TDG were shown to be significant factors. Significance of radiation and time for tumor growth agreed with the data. The interaction of blocks with 5TDG implied that the effect of 5TDG upon tumor growth varied between the two studies. ^

Degree

Ph.D.

Subject Area

Health Sciences, Radiology

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