STUDIES ON THE BIOSYNTHESIS AND DEGRADATION OF HYPOTHALAMIC RELEASING HORMONES
Abstract
A proline endopeptidase from rat brain was isolated and characterized. Conventional isolation techniques were employed. The enzyme is a monomeric protein with a molecular weight of approximately 70,000. It has an isoelectric point of pH 4.5 and a pH optimum of 7- 8.5. The proline endopeptidase from rat brain demonstrated a specificity toward proline containing peptides as compared with proline containing proteins. A synthetic oligonucleotide 5' d(TTCCAGAAGAA) was used as a primer for the AMV reverse transcriptase catalyzed synthesis of a cDNA to somatostatin RNA from catfish pancreas. This cDNA to somatostatin mRNA was partially sequenced using the chemical degradation sequencing methods. The cDNA fragment was then used to identify somatostatin containing clones from a library of catfish pancreas cDNA. Two clones identified by this procedure were sequenced to determine the entire structure of the DNA encoding a precursor to catfish pancreatic somatostatin-14.
Degree
Ph.D.
Subject Area
Biochemistry
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