Fundamentals, method development, and applications of paper spray ionization mass spectrometry
Abstract
Paper Spray is a novel ambient ionization source for mass spectrometry. This method, along with other ambient sources, is gaining popularity for its ease-of-use and the lack of need for sample preparation for in situ analysis. The work demonstrated in this dissertation covers the fundamentals, method development, and applications for paper spray. The novel fundamental research deepens the understanding of the methodology to make future improvements in method development. High resolution images and measurement of the paper spray droplet sizes using a Phase Doppler Anemometer indicate that ionization from cellulosic microfibers provides a unique environment to create charged spray droplets. Further support of the mechanism is shown in a study of the ion current, analyte response, and internal energy deposition. Applications were pursued including trace explosives detection, quantitative drug analysis from blood, and organic reactions. The utility of these methods is realized with the time and cost savings of rapid on-line extraction/analysis. Quantitation also shows that highly reproducible and accurate results better than ±10% were achieved when using isotopically-labeled internal standards. Paper spray has proved to be a powerful tool in conjunction with mass spectrometry and simplifies analysis of complex matrices and mixtures.
Degree
Ph.D.
Advisors
Cooks, Purdue University.
Subject Area
Chemistry|Analytical chemistry
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