The analysis of small organic molecules/biomolecules using ion/ion reactions in the gas phase with a quadrupole time-of-flight tandem mass spectrometer
Abstract
The use of a commercial quadrupole/time-of-flight (QqTOF) tandem mass spectrometer, (QSTAR XL, AB/Sciex) modified for ion/ion reaction studies, offers a powerful means for the accurate analysis of small organic molecules and large biological polymers such as alpha-glucosamines, proteins, peptides, and lignins. Gas-phase ion-ion chemistry and ion parking were used to concentrate precursor ions into an interested species. Ion activation was performed in MS/MS experiments followed by ion-ion reaction in order to simplify post-ion/ion spectrum interpretation. The projects include, for example, the application of electrospray and ion/ion chemistry to the study of the dissociation chemistry of multiply-charged macro-ions, and the combination of ion/ion chemistry and unimolecular dissociation chemistry for the rapid identification and characterization of proteins and peptides.
Degree
Ph.D.
Advisors
McLuckey, Purdue University.
Subject Area
Analytical chemistry
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