Physical interactions of membrane receptors of two human cancer cell lines

Jacob A Hale, Purdue University

Abstract

Using non-invasive single molecule labeling techniques, individual prostate specific membrane antigen (PSMA) and folate receptor (FR) membrane proteins were observed to diffuse randomly but interrupted by frequent periods during which the diffusion coefficient was less than experimental noise, termed Transient Anchorage. Under these near-native labeling conditions, no Transient Confinement was observed, a condition where the diffusion coefficient is reduced from the trajectory average but still above experimental noise, a condition observed for strongly interacting labeling procedures. The level of Transient Anchorage for PSMA proteins was proportional to the concentration of ligand in solution and suggests that ligation may induce fluid complex formation of receptor proteins. pted by frequent periods during which the diffusion coefficient was less than experimental noise, termed Transient Anchorage. Under these near-native labeling conditions, no Transient Confinement was observed, a condition where the diffusion coefficient is reduced from the trajectory average but still above experimental noise, a condition observed for strongly interacting labeling procedures. The level of Transient Anchorage for PSMA proteins was proportional to the concentration of ligand in solution and suggests that ligation may induce fluid complex formation of receptor proteins.

Degree

Ph.D.

Advisors

Ritchie, Purdue University.

Subject Area

Cellular biology|Molecular physics|Biophysics|Oncology

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