Interactions of vascular ECM macromolecules and their influence on SMC behavior

Katherine A Stuart, Purdue University

Abstract

Collagen is explored heavily as a biomaterial for tissue engineering applications. Type I collagen organization in the body is tissue specific, and this organization and the resulting mechanical properties are controlled by cells and other ECM molecules. This study examined materials composed of type I collagen, type III collagen, and the glycosaminoglycan chondroitin sulfate (CS) in order to more closely mimic natural environments for cell growth. The combination of these three components resulted in differences in the network’s resulting biochemical, structural, and mechanical cues. These changes in material properties occurred by combining these materials in physiologically relevant ratios of collagen to CS, and at physiological pH and ionic strength, making them directly applicable for use in tailoring biomaterials for tissue engineering applications. We found that SMCs cultured within these different matrices had increased remodeling capabilities and cell viability when types I and III collagen were combined in ratios most closely matched to the natural ECM surrounding SMCs in the medial layer of the vessel wall. The ability to control a material’s viscoelastic and structural characteristics, in combination with presenting relevant biological cues to cells, has important implications for all tissue engineering applications.

Degree

Ph.D.

Advisors

Panitch, Purdue University.

Subject Area

Biomedical engineering

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