Analgesic effect of bupivacaine eluting porcine small intestinal submucosa (SIS) in a ferret acute abdominal hernia defect model
Porcine small intestinal submucosa (SIS) is used as a biological graft for abdominal wall hernia repair to facilitate wound healing and increase local tissue strength. This prospective, randomized, double blinded study evaluated efficacy and duration of analgesia using bupivacaine adsorbed to SIS for repair of acutely created abdominal wall full thickness muscle/fascial defects in ferrets. Eighteen one year old, healthy male ferrets were randomly and equally assigned to three groups: (1) SIS with bupivacaine subjected to surgery, (2) SIS with no bupivacaine subjected to surgery, and (3) anesthesia only control group. Ferrets in groups 1 and 2 were anesthetized with butorphanol and sevoflurane for creation and repair of the abdominal body wall defect with SIS. Control ferrets were anesthetized in the same fashion for the same duration without surgery. Behavior and pain were evaluated in all ferrets using a visual analogue scale, heart and respiratory rates, algometer and palpometer measurements at various hour intervals for 96 hours after surgery. When pain reached a predetermined threshold, buprenorphine was used as a rescue analgesic. Plasma concentration of bupivacaine was analyzed using Liquid chromatography mass spectrometry/mass spectrometry (LC-MS/MS). Data was analyzed using Repeated Measures ANOVA and Kaplan-Meier Survival Analysis with p<0.05. While there was no statistical significance between groups with heart and respiratory rates, algometer and palpometer values for assessing pain, there was a physiological behavioral significant difference in these values. All ferrets in SIS alone group were rescued while only 33% of the SIS-bupivacaine group were rescued (p<0.01). Peak plasma concentrations of bupivacaine were in the range of 1.2 μg/mL. Bupivacaine adsorbed to SIS provided pain relief with no side effects observed in this ferret abdominal wall defect model.^
Jeff C. Ko, Purdue University.
Biology, Veterinary Science