Mechanical property and biocompatibility of PLLA coated DCPD composite scaffolds
Abstract
Dicalcium phosphate dihydrate (DCPD) cements have been used for bone repairdue to its excellent biocompatibility and resorbability. However, DCPD cements aretypically weak and brittle. To overcome these limitations, the sodium citrate used as asetting regulator and the coating of poly-L-lactide acid (PLLA) technique have beenproposed in this study. The first purpose of this thesis is to develop composite PLLA/DCPD scaffolds with enhanced toughness by PLLA coating. The second purpose is toexamine the biocompatibility of the scaffolds. The final purpose is to investigate thedegradation behaviors of DCPD and PLLA/DCPD scaffolds. In this experiment, DCPDcements were synthesized from monocalcium phosphate monohydrate (MCPM) and B-tricalcium phosphate (B-TCP) by using deionized water and sodium citrate as liquidcomponents. The samples were prepared with powder to liquid ratio (P/L) at 1.00, 1.25and 1.50. To fabricate the PLLA/DCPD composite samples, DCPD samples were coatedwith 5% PLLA. The samples were characterized mechanical properties, such as porosity,diametral tensile strength, and fracture energy. The mechanical properties of DCPDscaffolds with and without PLLA coating after the in vitro static degradation (day 1, week1, 4, and 6) and in vitro dynamic degradation (day 1, week 1, 2, 4, 6, and 8) wereinvestigated by measuring their weight loss, fracture energy, and pH of phosphate buffersolution. In addition, the dog bone marrow stromal stem cells (dBMSCs) adhesion onDCPD and PLLA/DCPD composite samples were examined by scanning electronmicroscopy.
Degree
M.S.B.M.E.
Advisors
Chu, Purdue University.
Subject Area
Biomedical engineering
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